Potential of Fungi Isolate as a Biological Control of White Root Disease (Rigidoporus sp.) on Rubber Plant
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Abstract
Rigidoporus Sp. is a white root fungus which is the main diseases problems on rubber plants in the wet and dry lands of South Kalimantan. An economically and ecologically beneficial control solution for this problem soil contagion was by using antagonistic fungi. This research was aimed to study the potential of rhizosphere and endophytic fungi in swampland, to control white root fungus on rubber plants. The research used exploratory method. Rhizospheric fungi were explored from swamp rubber plant roots at Pulau Damar village Hulu Sungai Utara District; endophytic fungi were isolated from leaves and jelutung swamps (Dyera lowii) which is rubber-like plants. The isolates found then were screened based on the percentage of inhibition using the dual culture method between pathogens and test isolates, also observed the interaction mechanism and viability test. The research in the laboratory was arranged using a Completely Randomized Design with one factor i.e. the type of antagonistic fungi. Five isolates which had the highest inhibitory power were isolates I13K4R, I7K3R, I1K2R, I6K2R, and I1E with successive inhibitions 95.00%, 83.00%, 76.50%, 62.50% and 53.00%. Thus, isolates have included potential antagonistic agents because they had the ability in space competition more than 50% and proven to be able to paralyze Rigidoporus. The viability test results showed that the isolate that had germination capability of more than 60% were respectively I13K4R 88.05%, I7K3R 87.33%, I1K2R 86.93%, I6K2R 72.85%, and I1E 74.78%.
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References
Amaria, W., Rita, H. dan Samsudin. 2015. Evaluasi jamur antagonis dalam menghambat pertumbuhan Rigidoporus lignosus penyebab penyakit jamur akar putih pada tanaman karet. Balai Penelitian Tanaman Industri dan Penyegar. J. TIDP. 2(1): 51-60.
Amaria, W., Taufiq, E. dan Harni, R. 2013. Seleksi dan identifikasi jamur antagonis sebagai agens hayati jamur akar putih (Rigidoporus microporus) pada tanaman karet. Balai Penelitian Tanaman Industri dan Penyegar. Buletin Ristri. 4 (1): 55-64.
Benitez, T., Rincon, A.M., Limon, M.C. and Codon, A.C. 2004. Biocontrol Mechanisms of Trichoderma strains. International Microbiology 7: 249-260.
Chen, Y.C and Ginette S-S. 2002. A rapid method for assessing the viability of fungal spores. Canadian Journal of Plant Pathology 24(2) : 230-232 DOI: 10.1080/07060660309507000
Dinas Perkebunan. 2016. Luas dan produksi pengusahaan komoditi perkebunan tanaman tahunan dan semusim. Laporan Triwulan Kabupaten/ Kota. Stastik Perkebunan Kalimantan Selatan Tahun 2016. Banjarbaru
DPP (Direktorat Perlindungan Perkebunan). 2014. Pedoman Uji Mutu Dan Uji Efikasi Lapangan Agens Pengendali Hayati (APH). Jakarta.
Farhana, A.H.K.F., Shamsul, B.A.R., Vu Thanh, T.A. and Zakaria, L. 2017. Morphological features of Rigidoporus microporus isolated from infected malaysian rubber clones. Malaysian Journal of Microscopy. 13: 17-23.
Gupta, G., Das, S.K., Rao, D.N. and Vasudevan, D.M. 2014. Effect of lecithin with vitamin-B complex and tocopheryl acetate on long term effect of ethanol induced immunomodulatory activities. J. Biol. 45(8): 681-683.
Heydari, A and Pessarakli, M. 2010. A Review on Biological Control of Fungal Plant Pathogens Using Microbial Antagonists. Journal of Biological Sciences 10(4): 273-290. DOI: 10.3923/jbs.2010.273.290
Jayasuriya, K.E. and Thennakoon, B.I. 2007. Biological control of Rigidoporus microporus, the cause of white root disease in rubber. Cey. J. Sci. 36(1): 9-16.
Kaewchai, S.L.F.C., Wang, H.K. and Soytong, K. 2010. Characterization of Rigidoporus microporus isolated from rubber trees based on morphology and ITS sequencing. Journal of Agricultural Technology. 6(2): 289-298.
Köhl, J. Kolnaar,R., and Ravensberg W.J.. 2019. Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy. Review Article. Front. Plant Sci., 19 July 2019 | https://doi.org/10.3389/fpls.2019.00845
Kullnig, C., Mach, R.L. Lorito, M. and Kubicek, C.P. 2000. Enzyme diffusion from Trichoderma atroviride (=T. harzianum P1) to Rhizoctonia solani is a prerequisite for triggering of Trichoderma ech42 gene expression before mycoparasitic contact. Microbial. 66(5): 2232-2239.
Mariana., Yusriadi. dan Ismed, S.B. 2016. Efektivitas Trichoderma indigenous dalam menekan pertumbuhan penyakit jamur akar putih pada tanaman karet. Prosiding Seminar Nasional Perhimpunan Fitopatologi Indonesia Pengendalian Penyakit Pada Tanaman Ramah Lingkungan II pada 27 Agustus 2016. Yogyakarta.
Nielsen, M. N. dan Winding, A. 2002. Microorganism as indicators of soil health. National Enviromental Research Institute, Denmark. Technical Report Vol. 388. http://pure.au.dk/portal/en/publications/microorganisms-as-indicators-of-soil-health(46bdc4f0-7e9d-11dda5a8000ea68e967b).html. Diakses tanggal 29 April 2019.
Novianti, R., Haryono, S., Ferry, F.K. dan Martanto, M. 2011. Analisis variabilitas genetik Rigidoporus microporus (jamur akar putih) pada karet dengan teknik PCR-RFLP fragment ITS. Jurnal Perlindungan Tanaman Indonesia. 7(1): 1-6.
Nurhayati, 2010. Senarai istilah-istilah mikolologi. UNSRI Press: Palembang.
Oku, S., Komatsu, A., Tajima, T., Nakashimada, Y. and Kato, J. 2012. Identification of chemotaxis sensory proteins for amino acids in Pseudomonas fluorescens Pf0-1 and their involvement in chemotaxis to tomato root exudate and root colonization. Microbes Environ. 27(4): 462-469.
Pal, K. K. and B. Mc.S. Gardener, 2011. Biological Control of Plant Pathogens. The Plant Health Instructor DOI: 10.1094/PHI-A-2006-1117-02.
Rahman, M.A., M. F. Begum and M. F. Alam. 2009. Screening of Trichoderma Isolates as a Biological Control Agent against Ceratocystis paradoxa Causing Pineapple Disease of Sugarcane Mycobiology 37(4):277-85
Semangun, H. 2008. Penyakit-penyakit tanaman perkebunan di Indonesia. Gadjah Mada University Press, Yogyakarta.
Shofiana, R.S., Liliek, S. dan Anton, M. 2015. Eksplorasi jamur endofit dan khamir pada tanaman cengkeh (Syzygium aromaticum) serta uji potensi antagonismenya terhadap jamur akar putih (Rigidoporus microporus). Jurnal HPT. 3(1): 75-83.
Simatupang, D.S. 2008. Berbagai mikroorganisme rhizosfer pada tanaman papaya (Carica papaya L.) di pusat kajian buah-buahan tropika (PKBT) IPB Desa Ciomas, Kecamatan Pasirkuda, Kabupaten Bogor, Jawa Barat. Skripsi. Institut Pertanian Bogor. Bogor.
Skidmore, A. M. and Dickinson, C. H. 1976. Colony interactions and hyphal interference between Septoria Nodorum and phylloplane fungi.Trans.Brit. Mycol.Soc. 66:57-64.
Soesanto, L. 2008. Pengantar pengendalian hayati penyakit tanaman. Rajawali Press: Jakarta
Surtikantis dan Yasin, M. 2009. Keefektifan entomopatogenik Beuveria bassiana Vuill. dari berbagai media tumbuh terhadap Spodoptera litura F. (Lepidoptera : Noctuidae) di Laboratorium. Prosiding Seminar Nasional Serealia, 27 September 2009. Balai Penelitian Tanaman Serealia.
Ubogu, M. 2013. Assessment of root zone mycoflora of three Havea brasiliensis (Rubber) clones at akwete plantations and their in vitro growth inhition of Rigidoporus lignosus. European Journal of Experimental Biology. 3(2): 618-623.
Van Agtmaal, M. van Os, G. J. Hol W.H.G., Hundscheid M.P.J., Runia, W.T. Hordijk, C.A. and de Boer W.. 2015. Legacy effects of anaerobic soil disinfestation on soil bacterial community composition and production of pathogen-suppressing volatiles. Front. Microbiol., 10 July 2015. https://doi.org/10.3389/fmicb.2015.00701
Waluyo, T.K., I. Wahyudi, G. Santosa. 2012. Pengaruh Metode Dan Arah Sadap Terhadap Produksi Getah Jelutung Hutan Tanaman Industri . Jurnal Penelitian Hasil Hutan 30 (4): 3001-311.